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Repeated measurements of endocrine markers were analyzed with linear mixed-effects (LME) models, having drug condition (placebo, oral cannabis, smoked cannabis, and vaporized cannabis), session number (1, 2, 3, and 4), blood sampling time-point (T1, T2, T3, and T4), and drug time-point interaction as fixed effects, individual subjects as a random effect (random intercept and slope), and each hormone as the outcome

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Optimization of experimental parameters Next, to optimize the assay conditions, we investigated the effects of BHZ concentration (0150 M) and duration of labeling (2472 h) on A7r5 cells that overexpressed LOXL2 via adenoviral delivery (Fig

The reaction was kept in the dark for 1 h, and absorbance was taken at 390 nm

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